Species: Goat (Capra hircus)

Country of 1st Author: China
Year: 2018

Institute	Yangzhou University Yangzhou, Jiangsu 225009 CN
Editing Method	Embryo edit
SDN Type	SDN-1
Delivery	A mixture of in vitro transcribed sgRNA (10 ng/µl)and Cas9 mRNA (40 ng/µl)was injected into the cytoplasm ofpronuclear stage embryos.
Foreign DNA	No
Undergone Regulatory Process	No

Target Gene(s)

Gene Name	Gene Trait	Trait Category	NGT	Methods/Remarks
MSTN Myostatin	Double muscle	Yield	CRISPR Cas9 (spCas9)	A mixture of in vitro transcribed sgRNA (10 ng/µl)and Cas9 mRNA (40 ng/µl)was injected into the cytoplasm ofpronuclear stage embryos.

References, Documents, and Links

• Use of CRISPR/Cas9 technology efficiently targetted goat myostatin through zygotes microinjection resulting in double-muscled phenotype in goats