Species: Pig (Sus scrofa domesticus)

Country of 1st Author: United States
Year: 2022

Breed Strain	Landrace/Large White/Duroc/Synthetic and subsequent crosses
Commercial Name	Same as current commercial line names
Commercial Use	Standard breeding programs as permitted
Institute	Genus PLC US
Editing Method	Embryo edit
SDN Type	SDN-1
Delivery	RNP
Foreign DNA	No
Undergone Regulatory Process	No

Target Gene(s)

Gene Name	Gene Trait	Trait Category	NGT	Methods/Remarks
CD163 Cluster of Differentiation 163	Resistance against Porcine reproductive and respiratory syndrome (PRRS)	Biotic Stress	CRISPR Cas9 (spCas9)	CRISPR/Cas9 protein complex with two gRNAs leading to double-strand DNA break and repair

References, Documents, and Links

• Mark Cigan: Commercializing PRRS Virus Resistant Pigs
• Pigs Lacking the Scavenger Receptor Cysteine-Rich Domain 5 of CD163 Are Resistant to Porcine Reproductive and Respiratory Syndrome Virus 1 Infection