Species: Cattle (Bos taurus)

Country of 1st Author: United States
Year: 2016

Institute	University of Washington Pullman, WA US
Editing Method	Cloned cell line
SDN Type	SDN-2
Delivery	Three pairs of zinc-finger nucleases (ZFN) were custom-made (Sigma? Aldrich) to target exon 2 of bovine ITGB2 (integrin subunit beta 2) that encodes the signal peptide of CD18. The ZFN pair with the highest activity in the MEL-1 assay (Sigma) was used for gene targeting. Fetal fibroblasts were subjected to nucleofection (Lonza) using the pair of ZFN mRNAs along with a singlestranded oligonucleotide
Foreign DNA	No
Undergone Regulatory Process	No

Target Gene(s)

Gene Name	Gene Trait	Trait Category	NGT	Methods/Remarks
CD18 Cluster of Differentiation 18	Resistance against Mannheimia haemolytica leukotoxin-resistant cattle	Biotic Stress	ZFN	Fetal fibroblasts were subjected to nucleofection using the pair of ZFN mRNAs along with a single stranded oligonucleotide

References, Documents, and Links

• Precise gene editing paves the way for derivation of Mannheimia haemolytica leukotoxin-resistant cattle