Species: Cattle (Bos taurus)

Country of 1st Author: Russia
Year: 2021

Institute	Ernst Federal Science Center for Animal Husbandry Podolsk RU
Editing Method	Cloned cell line
SDN Type	SDN-1
Delivery	To obtain individual colonies of bovine GE fibroblasts, the early-passage fibroblasts were electroporated with a mixture of plasmids encoding Cas9 and gRNA,aimed at inactivating the BLG
Foreign DNA	No
Undergone Regulatory Process	No

Target Gene(s)

Gene Name	Gene Trait	Trait Category	NGT	Methods/Remarks
BLG β-Lactoglobulin	Knockout of beta-lactoglobulin for hypoallergenic milk	Hypoallergenic	CRISPR Cas9 (spCas9)

References, Documents, and Links

• Production of a Cloned Offspring and CRISPR/Cas9 Genome Editing of Embryonic Fibroblasts in Cattle