Species: Silkworm (Bombyx mori)

Country of 1st Author: China
Year: 2020

Institute	Southwest University 400715 Chongqing CN
Editing Method	Other
SDN Type	SDN-3
Delivery	FnCas12a × gIE1 and SpCas9 × sgIE1 transgenic hybrid lines of silkworm were evaluated the gene-editing efficiency of different systems at the same target site. The antiviral ability was improved using the FnCas12a system in transgenic silkworm.
Foreign DNA	Yes
Undergone Regulatory Process	No

Target Gene(s)

Gene Name	Gene Trait	Trait Category	NGT	Methods/Remarks
BmNPV Bombyx mori nuclear polyhedrosis virus	Resistance to nucleopolyhedrovirus (BmNPV)	Biotic Stress	CRISPR Cas12a (Cpf1)	Three three Cas12a orthologs nucleases, AsCas12a, FnCas12a, and LbCas12a, were evaluated for their editing efficiencies and antiviral abilities in silkworm

References, Documents, and Links

• CRISPR/Cas12a Mediated Genome Editing Enhances Bombyx mori Resistance to BmNPV