Species: Sterlet (Acipenser ruthenus)

Country of 1st Author: China
Year: 2018

Institute	Chinese Academy of Sciences, Shanghai CN
Editing Method	Embryo edit
SDN Type	SDN-1
Delivery	Each gRNA was transcribed and mixed with Cas9 nuclease. The Cas9 nuclease/gRNA at was microinjected into the animal pole of one-cell stage embryos.
Remarks	A marker enhanced green fluorescent protein (EGFP) was included in this experiment
Foreign DNA	Yes
Undergone Regulatory Process	No

Target Gene(s)

Gene Name	Gene Trait	Trait Category	NGT	Methods/Remarks
ntl no tail	no tail gene (ntl), a key gene participating in the formation of normal tail, was used as a target for ribonuclease (RNase) P-mediated gene disruption in zebrafish in vivo	Other	TALEN	Three gRNAs were designed to target the first exon of the no tail (ntl) gene in sterlet

References, Documents, and Links

• Efficient Gene Transfer and Gene Editing in Sterlet (Acipenser ruthenus)