Species: Pig (Sus scrofa domesticus)

Country of 1st Author: Taiwan
Year: 2020

Institute	Agricultural Technology Research Institute Xiangshan Dist., Hsinchu TW
Editing Method	Cloned cell line
SDN Type	SDN-2
Delivery	A pRSAD2-KI pig that constitutively overexpressed the pRSAD2 was cloned , viral challenge results indicated that fibroblasts isolated from the pRSAD2-KI pig reduced CSFV infection.
Foreign DNA	No
Undergone Regulatory Process	No

Target Gene(s)

Gene Name	Gene Trait	Trait Category	NGT	Methods/Remarks
pRSAD2	Resistance against classic swine fever virus (CSFV) and pseudorabies virus (PRV)	Biotic Stress	CRISPR Cas9 (spCas9)	site-specific insertion of the porcine RSAD2 gene (pRSAD2) at the porcine ROSA26 (pROSA26) locus, generating pRSAD2 gene knock-in (pRSAD2-KI)

References, Documents, and Links

• Generation of pRSAD2 gene knock-in pig via CRISPR/Cas9 technology