Species: Pig (Sus scrofa domesticus)

Country of 1st Author: United States
Year: 2016

Institute	University of Missouri Columbia, Missouri US
Editing Method	Embryo edit
SDN Type	SDN-1
Delivery	the CRISPR/Cas9 system was delivered into in vitro produced presumptive porcine zygotes,
Remarks	Guide RNAs were designed to regions within exon 7 of CD163
Foreign DNA	No
Undergone Regulatory Process	No

Target Gene(s)

Gene Name	Gene Trait	Trait Category	NGT	Methods/Remarks
CD163 Cluster of Differentiation 163	Resistance against Porcine reproductive and respiratory syndrome (PRRS)	Biotic Stress	CRISPR Cas9 (spCas9)
CD1D	This gene encodes a divergent member of the CD1 family of transmembrane glycoproteins, which are structurally related to the major histocompatibility complex (MHC) proteins and form heterodimers with beta-2-microglobulin.	Biotic Stress	CRISPR Cas9 (spCas9)

References, Documents, and Links

• Gene-edited pigs are protected from porcine reproductive and respiratory syndrome virus
• Use of the CRISPR/Cas9 system to produce genetically engineered pigs from in vitro-derived oocytes and embryos