Species: Pig (Sus scrofa domesticus)

Country of 1st Author: China
Year: 2019

Breed Strain	Landrace
Institute	State Key Laboratory for Agrobiotechnology, College of Biological Sciences, China Agricultural University Beijing CN
Editing Method	Cloned cell line
SDN Type	SDN-3
Delivery	pX330-501 vector and a donor vector. pX330-501 vector was designed to target exon 7 of CD163
Foreign DNA	Yes
Undergone Regulatory Process	No

Target Gene(s)

Gene Name	Gene Trait	Trait Category	NGT	Methods/Remarks
CD163 Cluster of Differentiation 163	Resistance against Porcine reproductive and respiratory syndrome (PRRS)	Biotic Stress	CRISPR Cas9 (spCas9)	substituted exon 7 of porcine CD163 with the corresponding exon of human CD163-like 1 (hCD163L1)

References, Documents, and Links

• Generation of Pigs Resistant to Highly Pathogenic-Porcine Reproductive and Respiratory Syndrome Virus through Gene Editing of CD163