Species: Pig (Sus scrofa domesticus)

Country of 1st Author: United Kingdom
Year: 2018

Breed Strain	Large white / landrace / duroc
Institute	University of Edinburgh (Roslin Institute) with Genus UK
Editing Method	Embryo edit
SDN Type	SDN-1
Delivery	Microinjection of RNP
Foreign DNA	No
Undergone Regulatory Process	No

Target Gene(s)

Gene Name	Gene Trait	Trait Category	NGT	Methods/Remarks
CD163 Cluster of Differentiation 163	Resistance against Porcine reproductive and respiratory syndrome (PRRS)	Biotic Stress	CRISPR Cas9 (spCas9)	Exon 7 deletion to remove protein domain 5 from the expressed protein. Without this protein domain the PRRS virus cannot establish an infection

References, Documents, and Links

• Pigs Lacking the Scavenger Receptor Cysteine-Rich Domain 5 of CD163 Are Resistant to Porcine Reproductive and Respiratory Syndrome Virus 1 Infection
• Precision engineering for PRRSV resistance in pigs: Macrophages from genome edited pigs lacking CD163 SRCR5 domain are fully resistant to both PRRSV genotypes while maintaining biological function