Species: Olive flounder (Paralichthys olivaceus)

Country of 1st Author: South Korea
Year: 2019

Institute	National Institute of Fisheries Science Busan 46083 KR
Editing Method	Embryo edit
SDN Type	SDN-1
Delivery	The fertilized embryos were injected on a microinjection plate. gRNAs were microinjected at a final concentration of 1?µg/µL together with 1–1.5?µg/µL Cas9 mRNA, 200?mM KCl, and phenol red solution into 1-cell or 2-cell stage embryos
Foreign DNA	No
Undergone Regulatory Process	No

Target Gene(s)

Gene Name	Gene Trait	Trait Category	NGT	Methods/Remarks
MSTN Myostatin	Double muscle	Yield	CRISPR Cas9 (spCas9)

References, Documents, and Links

• CRISPR/Cas9-mediated myostatin disruption enhances muscle mass in the olive flounder Paralichthys olivaceus