Species: Sheep (Ovis aries)

Country of 1st Author: China
Year: 2016

Institute	Northwest A&F University Yangling 712100, Shaanxi CN
Editing Method	Embryo edit
SDN Type	SDN-1
Delivery	co-injecting one-cell-stage embryos with Cas9 mRNA and RNA guides targeting BCO2.
Foreign DNA	No
Undergone Regulatory Process	No

Target Gene(s)

Gene Name	Gene Trait	Trait Category	NGT	Methods/Remarks
BCO2 beta-carotene oxygenase 2	This gene encodes an enzyme which oxidizes carotenoids such as beta-carotene during the biosynthesis of vitamin A.	Other	CRISPR Cas9 (spCas9)	Cas9 mRNA and two sgRNAs targeting exon 2 of the BCO2 gene were microinjected intracytoplasmatically into one cell stage sheep zygotes

References, Documents, and Links

• Biallelic ß-carotene oxygenase 2 knockout results in yellow fat in sheep via CRISPR/Cas9