Species: Sheep (Ovis aries)

Country of 1st Author: China
Year: 2020

Institute	Northwest A&F University Yangling 712100, Shaanxi CN
Editing Method	Embryo edit
SDN Type	SDN-1
Delivery	BEmax mRNA (25 ng µL-1) and sgRNA (10 ng µL-1) were coinjected into the cytoplasm of the collected zygotes using an Eppendorf FemtoJet system
Foreign DNA	No
Undergone Regulatory Process	No

Target Gene(s)

Gene Name	Gene Trait	Trait Category	NGT	Methods/Remarks
BMPR1B Bone morphogenetic protein receptor type-1B	Encodes a member of the bone morphogenetic protein (BMP) receptor family of transmembrane serine/threonine kinases	Reproduction	DNA base editor	Adenine base editors (ABE) ABEmax is one of the latest versions of ABE, which consists of a catalytically-impaired nuclease and a laboratory-evolved DNA-adenosine deaminase.

References, Documents, and Links

• Highly efficient generation of sheep with a defined FecB^B mutation via adenine base editing