Downregulation of C3'H Gene in Rice Alters Cell Wall StructuresJune 20, 2018
p‐Coumaroyl ester 3‐hydroxylase (C3ˊH) is a key enzyme involved in the synthesis of lignin, a major component in vascular plants' secondary cell walls. Although the crucial role of C3ˊH in have been investigated, there is limited information available for its role in monocotyledonous grass species. The team of Yuri Takeda from Kyoto University in Japan studied the effect of C3ˊH‐deficiency on the structure and properties of grass cell walls.
C3ˊH‐knockdown lines generated via RNAi gene silencing, with approximately 0.5% of the normal expression level, reached maturity and set seeds. Meanwhile, C3ˊH‐knockout rice mutants generated via CRISPR-Cas9 mutagenesis were severely dwarfed and sterile.
Analysis of the mature C3ˊH‐knockdown RNAi lines revealed that their lignin were enriched in p‐hydroxyphenyl (H) units and had reduced levels of the normally dominant guaiacyl (G) and syringyl (S) units. Suppression of C3ˊH also resulted in substantial reduction in cell wall cross‐linking. The C3ˊH‐suppressed rice exhibited enhanced biomass saccharification.
These data demonstrate that C3ˊH expression is an important determinant of lignin content and composition as well as the degree of cell wall cross‐linking.
For more information, read the article in The Plant Journal.
See more articles:
News from Around the World
- Study Shows Farm Income and Production Impacts of GM Crop Technology from 1996 to 2016
- AATF Receives Multi-million Grant for Bt Maize Commercialization in Africa
- Agriculture Minister Proposes a Law to Regulate Seeds and Seed Varieties in Namibia
- High-protein Maize Shows Resistance to Parasitic Weed
- Critical Plant Gene Takes Detour that Could Boost Biofuel Yields
- HarvXtra® Alfalfa Trait Deregulated in Argentina
- Australian OGTR Invites Comments on GM Canola Field Trials
- "Pink Bollworm Management Strategy" Book Now Available in Gujarati
- Plant Defense Genes Rewired to Reduce Crop Waste
- New Type of Photosynthesis Discovered
- GmNMH7 Inhibits Root and Nodule Development in Soybean
- IbSnRK1 Gene Improves Starch Content and Quality in Sweet Potato
Plant Breeding Innovations
- Downregulation of C3'H Gene in Rice Alters Cell Wall Structures
- Scientists Find Candidate Gene for Improving Potassium Deficiency Tolerance in Rice
- APETALA2 Gene Key to Improving Seed Production in Brassica Species
- Researchers Use CRISPR to Improve Nutrient Use Efficiency of Rice
- GPMB 2018
- World Food Day 2018 Poster Contest
Subscribe to CBU: