Researchers Apply CRISPR-Cas9 on Cacao LeavesFebruary 28, 2018
Cacao producers suffer significant losses due to a variety of pathogens. Development of disease resistant cacao varieties is an essential strategy to combat this threat, but is limited by sources of genetic resistance and the slow generation time of this crop. Andrew Fister of Pennsylvania State University and colleagues present the first application of CRISPR-Cas9 genome editing technology in cacao leaves and cotyledon cells.
As a proof of concept, Fister's team targeted the cacao Non-Expressor of Pathogenesis-Related 3 (TcNPR3) gene, a potential suppressor of defense response. The team later identified the presence of mutations in ~30% of TcNPR3 copies in the treated tissues. The edited tissue exhibited an increased resistance to the cacao pathogen Phytophthora tropicalis. Downstream defense genes were also found to be upregulated. Analysis of off-target mutations did not reveal unwanted mutations.
These results confirm the function of NPR3 as a repressor of the cacao immune system and demonstrate the application of CRISPR-Cas9 as a genomic tool for cacao. Further studies will test the effectiveness of this tool at a whole plant level.
For more information, read the article in Frontiers in Plant Science.
See more articles:
News from Around the World
- WEMA Gives Hope for Farmers Battling Armyworm in Kenya
- Ghana CSIR Affirms Safety of GM Crops
- Research Reveals New Approach to Improve Nitrogen Use, Enhance Yield, and Promote Flowering in Rice
- Researchers Monitor Photosynthesis in Soybean Using Invisible Light
- Scientists Discover Secret of How to Triple Number of Sorghum Grains
- India Launches Massive Pink Bollworm Awareness Campaign
- Increasing Sugar Yield and Biomass Production in Arabidopsis Discovered by VIB Scientists
- Land Plants Evolved 100 Million Years Earlier than Thought
- Study Shows How Plants Use 'Baits' to Trap Pathogens
- RNAi-mediated Knockdown of GmFAD2-1B Improves Oil Quality in Soybean
- Rice Gene Confers Citrus Canker Resistance to Transgenic Mandarin
Plant Breeding Innovations
- CRISPR-Cas9 Used in Reverse Genetics Studies of Parasponia
- lncRNA1459 Knock-out Alters Tomato Fruit Ripening
- Researchers Apply CRISPR-Cas9 on Cacao Leaves
- Transgenerational CRISPR-Cas9 Induces Multiplex Gene Editing in Wheat
Beyond Crop Biotech
- Researchers Unlock Genetic Code of the Brightest and Most Vibrant Colors in Nature
- BIO Asia International Conference
- Plants, Genes, and Agriculture Book
Subscribe to CBU: