CRISPR-Cas9-Mediated Deletion of a Target Gene in Indica Rice

CRISPR-Cas9 has been widely used to produce gene-specific edits in both mammalian and plant systems. Most modifications in plants have been small insertions or deletions, with only a few large target gene deletions, especially for indica rice.

The team of Ying Wang from Syngenta Biotechnology China designed several CRISPR sgRNAs and successfully deleted DNA fragments in the DENSE AND ERECT PANICLE 1 (DEP1) gene in the elite Indica rice line IR58025B. The team achieved up to 2% mutation frequency for a 430 bp target and a 9% frequency for the 10 kb target among T0 events.

Improvements in yield-related traits, such as dense and erect panicles and reduced plant height, were observed in dep1 homozygous T0 mutant plants produced via CRISPR-Cas9.

For more on this study, read the article in Plant Cell Reports.


 

This article is part of the Crop Biotech Update, a weekly summary of world developments in agri-biotech for developing countries, produced by the Global Knowledge Center on Crop Biotechnology, International Service for the Aquisition of Agri-Biotech Applications SEAsiaCenter (ISAAA)

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