Heat-shock Inducible Genome Editing System with Natural Promoter Knockouts Gene in Plants
August 16, 2023| |
Researchers from Shanxi University and the Research Institute of Big Data Science and Industry in China were successful in editing target genes using both natural and synthetic heat-inducible promoters via CRISPR-Cas9. Further investigation concluded that the natural heat-inducible genome editing system was more successful at inactivating genes at any developmental stage of a plant.
The first system the scientists developed was composed of a natural heat-inducible promoter from the protein HSP18.2 of Arabidopsis thaliana, while the other was a synthetic heat-inducible promoter heat shock-response element HSE-COR15A that acted as the driver of Cas-9. Both systems underwent cyclic or continuous heat-shock treatments during the seedling and bolting stages of the Arabidopsis plant. Confirmatory tests, including PCR, revealed that HSP18.2 showed higher Cas-9 levels compared to HSE-COR15A, and that the HSP18.2 system resulted in highly efficient targeted mutagenesis with definite phenotypic outcomes.
The scientists demonstrated that heat shock-induced genome editing system can be used to conditionally knockout target genes with high efficiency and accuracy, and that the system can analyze gene function at any plant developmental stage.
More details in The Plant Genome.
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