Melon Transformation Made Easier and More Efficient
July 12, 2023| |
Scientists have made significant progress in improving the transformation efficiency of melon, a process that allows genes to be transferred into melon plants. The optimized protocol, along with the utilization of the CRISPR-Cas9-mediated cytosine base editor, provides a reliable framework for functional gene engineering in melon.
In the past, melon transformation was a complicated and inefficient process. However, the reported protocol makes it easier and more efficient to transfer genes into melon plants. The protocol involves immersing the explants in the inoculation medium for 20 minutes, using filter paper, 10 mM 2-(N-morpholino)-ethanesulfonic acid (MES), and a temperature of 24°C. The use of ethylene inhibitors and absorbers also helps to improve the transformation efficiency.
The optimized protocol has been used to generate stable gene-edited melon lines using the CRISPR-Cas9-mediated cytosine base editor. This allows for precise editing of melon genes, which could be used to improve melon traits such as taste, nutrition, and disease resistance. These advancements have the potential to revolutionize melon breeding and crop improvement.
Read the open-access article in the International Journal of Molecular Sciences.
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