Expression Green Fluorescent Protein and Cry1Ac in Transgenic Brassica spp.
July 1, 2011| |
The green fluorescent protein (GFP) has been used as a visual marker to understand biological structures and function at different levels. Chinese Academy of Agricultural Sciences scientist L. Lei and colleagues used this glowing protein to investigate the persistence and stability of a transgene that codes for Cry1Ac insecticidal protein. They used hybrids of Brassica napus and Brassica juncea (oilseed rape and Chinese mustard) and backcross offsprings containing Bt genes and GFP. The GFP marker gene served as a quantitative marker and indicator of Bt transgene expression.
Concentration of the Bt protein was found to be stable in all stages and generations of wild Chinese mustard hybrids and backcross offsprings. On the other hand, the concentration of Cry1Ac was found to be lower in the second backcross products, compared to the parent and first backcross progeny. Results also showed that the intensity of light exhibited by the GFP was significantly correlated to Cry1Ac concentration at flowering stage and pod formation stage in both oilseed rape and backcross offsprings. Thus, GFP could be a used as a reliable marker for Bt protein in backcross of Chinese mustard to facilitate the detection of gene flow during biosafety procedures.
Annals of Applied Biology subscribers can view the complete article here: http://onlinelibrary.wiley.com/doi/10.1111/j.1744-7348.2011.00486.x/full.
| |
Biotech Updates is a weekly newsletter of ISAAA, a not-for-profit organization. It is distributed for free to over 22,000 subscribers worldwide to inform them about the key developments in biosciences, especially in biotechnology. Your support will help us in our mission to feed the world with knowledge. You can help by donating as little as $10.
-
See more articles:
-
News from Around the World
- Partners Cooperate on Food Security Issues
- Jose Graziano da Silva of Brazil Elected FAO Director-General
- Annan Warns Hunger Could Become Permanent Disaster
- Scientists: Stem Rust Resistant Wheat Could Be Unveiled Soon
- Ghana Biosafety Bill Passed into Law
- U.S. Subcommittee Highlights Importance of Agribiotech to Feed the Growing Population
- Soybean Event MON87701 Safe for Livestock Feed, Says CFIA
- Improving Food Safety of Potato Varieties
- USDA Announces Decision to Deregulate Genetically Engineered Corn Seed
- Uruguay's CGR approved Five Maize Biotech Events
- Canola Biodiesel Reaches Milestone
- India Paying High Cost by Not Adopting GM Crops
- China Ratifies 7 GM Crops
- Congress on Genetic Engineering, Food Security, and Sustainable Development Concluded in Iran.
- China Discusses Importance of Biotechnology
- Workshop on Socio-Cultural Consideration, Biosafety, Biotechnology and Decision-making in Indonesia
- Scientific Research Essential for Agriculture in Vietnam
- Biotech Netmapping Workshop for Policymakers in Indonesia Concluded
- The Asia-Pacific Region Needs "New Agriculture"
- EU Adopts Regulation Harmonizing Controls for Non-authorized GM Material in Feed
- Global Plant Database Set to Promote Biodiversity Research and Earth-System Sciences
-
Research Highlights
- Mutations in Phytoene Synthase 1 Gene Alters Carotenoid Production
- Scientists Reveal the Effects of Bt Crops on Spiders
- Expression Green Fluorescent Protein and Cry1Ac in Transgenic Brassica spp.
-
Beyond Crop Biotech
- Regulation Blocking Genetically-engineered Food Animal Development, Report Finds
- Genome of Leaf-cutting Ants Reveal Clues on their Farming Behavior
-
Announcements
- 2012 World Potato Congress, Edinburgh, Scotland
-
Resources
- FAO Book on "Biotechnologies for Agricultural Development"
- FAO Biosafety Resource Book
-
Read the latest: - Biotech Updates (April 17, 2024)
- Gene Editing Supplement (April 10, 2024)
- Gene Drive Supplement (February 22, 2023)
-
Subscribe to BU: - Share
- Brassica spp.&url=https://www.isaaa.org/kc/cropbiotechupdate/article/default.asp?ID=8054" target="_blank" class="tweetbutton button100"> Tweet