Hybrid Rubisco of Tomato Large Subunits and Tobacco Small Subunits are Functional in Tobacco PlantsFebruary 18, 2011
During photosynthesis, plants convert carbon dioxide into sugar precursor through the activity of an enzyme called ribulose-1,5-biphosphate carboxylase/oxygenase (Rubisco). Rubisco has been known as the most important enzyme not just in photosynthesis but also in biomass productivity and carbon dioxide sequestration. However, Rubisco has very low catalytic efficiency, using only 20 to 70 carbon dioxide molecules per second. This led scientists to improve the activity of the enzyme Rubisco, but most studies on improving its functionality have been unsuccessful.
Xing-Hai Zhang from Florida Atlantic University, together with other scientists, created two lines of tobacco plants with rbcL gene coming from tomato with the objective of improving the activity of Rubisco. The first line, labeled as plant LLS2, contains Rubisco with the small subunit from tobacco and Q437R large subunit. The second line is called plant LLS4, with a hybrid Rubisco from tobacco small subunit, and tomato large subunit.
According to the results, plant LLS2 has similar phenotype with the wild type; while plant LLS4 exhibited decreased chlorophyll and Rubisco levels, lower photosynthesis rates and biomass, especially during the early development stages. However, plant LLS4 was able to achieve reproductive maturity with similar characteristics with the wild type. Both plant lines also exhibited comparable carboxylase activity and RuBP affinity as in the wild type. The hybrid Rubisco of tomato large subunit and tobacco small subunit can efficiently drive photosynthesis to support growth and reproduction of tobacco plants.
Read more about the results and discussions at http://dx.doi.org/10.1016/j.plantsci.2010.11.001.
The Crop Biotech Update is a weekly newsletter of ISAAA, a non-for-profit organization. The CBU is distributed for free to over 23,000 subscribers worldwide to inform them about the key developments in biosciences, especially in agricultural biotechnology. Your support will help us in our mission to feed the world with knowledge. You can help by donating as little as $10.
See more articles:
News from Around the World
- Reducing Poverty by Growing Fuel and Food
- New Rice Variety Could Ease Mozambique's Grain Supply
- Plants Cloned as Seeds
- Decreased Lignin Production in Switchgrass Boosts Biofuel Yield
- Using Genetics to Build a Better Tomato
- USDA Approves Corn Amylase Trait
- Philippine President Open to Array of Safe Agricultural Technologies
- NAST President and University Chancellor Condemn Attack on Bt Eggplant Field Trial
- Farmers Voice Should Be Heard, Bt Eggplant Benefits at Stake
- Professor Warns Health and Environmental Hazards of Excessive Insecticide Use, Bt Eggplant - a Promising Technological Development
- Biofortification Highlighted at Recent 2020 Conference
- IFPRI Research Delinks Bt Cotton and Farmer Suicides in India
- Training for Pakistan Farmers
- UAF to Set Up International Agriculture Center
- Turkey Approves GM Crops for Feed
- Agri Committee Urges Commission to Rethink Zero Tolerance on GM in Feed Imports
- EC-JRC Published 14 New Summary Notifications
- New UK Center to Address Production and Sustainability Challenges
- Detection of Causal Organism of Bacterial Disease of Onion
- Evaluation of the Brown Planthopper Resistance Genes in Hybrid Rice
- Scientists Evaluate Production of Allelochemicals by GE Oilseed Rape
- Hybrid Rubisco of Tomato Large Subunits and Tobacco Small Subunits are Functional in Tobacco Plants
Beyond Crop Biotech
- GM Mosquitoes Deployed to Control Asia's Dengue Fever
- US FDA Evaluating GE Salmon for Release
- World Congress on Biotechnology
- DBT Calls for Sustainable Crop Production Research Proposals
- Canadian Rising Stars in Global Health
- Australia Awards for Africa
Subscribe to CBU: