Identification of Sugarcane Genes Responsive to Ethanol Application
August 24, 2007| |
One of the most important challenges in plant biotechnology is the control of recombinant gene expression through a simple, inexpensive, efficient and safe activating mechanism. The activation of transgenic traits should only be when it is needed to prevent energy waste and avoid undesirable effects in plants. A number of chemical compounds control gene expression in plants. Ethanol is a suitable inducer since it is biodegradable, easy to apply, safe, and cheaper than other commonly used inducers.
cDNA microarray technology was utilizedt to identify transcript changes in sugarcane leaves exposed to ethanol. A gene which codes for ERD4 protein was found to be strongly induced by ethanol. Currently, researchers are conducting the identification and characterization of the promoter region of this gene. This study will facilitate gene cloning and its utilization in developing transgenic plants. Diversity determination studies are also being conducted to the ethanol responsive gene in different sugarcane varieties.
The complete paper published by the Journal Plant Cell Reports is available to subscribers at http://www.springerlink.com/content/b234l71v441121q6/fulltext.html
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