High Throughput Plant Gene Expression Analysis
July 13, 2007| |
A technique used by the biomedical community was demonstrated to have possible applications in studying gene expressions in plants. The application utilizes a microplate-based high-throughput array-based gene expression system, called qNPA, which may have advantages over conventional microarrays due to lower cost, higher reproducibility and accuracy.
The group of Richard Kris, who reviewed results of their tests on Arabidopsis thaliana, said that the qNPA platform may speed up gene-based discovery and development programs as it is capable of providing accurate dose-response. They likewise dispelled the premise that the expression system is difficult to use in plants because of the tough cell wall which impedes homogenization and cell breakage and also due to the presence of plant secondary compounds, which affects homogenization.
The open access article published in Plant Physiology can be viewed at http://www.plantphysiol.org/cgi/content/abstract/144/3/1256.
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