Transposition-Based Plant Transformation
February 23, 2007| |
Among the public concerns on genetic transformation is the permanent introduction of bacterial, viral, and synthetic DNA into the genomes of food crops. A new method of genetically modifying plants that does not require bacterial DNA inserted into their genomes has been reported by Hua Yan and Caius Rommens of the J.R. Simplot Company in Idaho.
Yan and Rommens used Agrobacterium T-DNAs containing both a maize Dissociation (Ds) element and Activator-Transposase (Ac-Tpa) gene to deliver the desired DNA sequence to potato stem explants. The method was found effective in the transformation of ‘Ranger Russet’ potato variety. Single-copy and backbone-free transformation events were observed at a rate that is a bit lower (2.5-fold) than the regular T-DNA transformation of potato.
The abstract published by the journal Plant Physiology, with links to the full paper for subscribers, is accessible at http://www.plantphysiol.org/cgi/content/abstract/143/2/570
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