DNA-free Genome Editing of Bread Wheat using CRISPR/Cas9 Ribonucleoprotein ComplexJanuary 25, 2017
Studies have been made to optimize the CRISPR/Cas9 system for crop breeding. The main reasons for these studies are the avoidance of transgene integration and reduction of off-target mutations. Zhen Liang of the Chinese Academy of Sciences aimed to develop an efficient genome editing method for bread wheat using CRISPR/Cas9 ribonucleoproteins (RNPs).
The developed protocol takes only seven to nine weeks. Four to five independent mutants were produced from 100 immature wheat embryos. Further analysis revealed much lower chance of off-target mutations in RNP-mediated genome editing than with CRISPR/Cas9 DNA. Furthermore, no off-target mutations were detected in the mutant plants.
Since no foreign DNA is used in CRISPR/Cas9 RNP-mediated genome editing, the mutants obtained were completely transgene-free. This method could be widely applicable for producing genome-edited crops.
For more information, read the article in Nature Communications.
Biotech Updates is a weekly newsletter of ISAAA, a not-for-profit organization. It is distributed for free to over 22,000 subscribers worldwide to inform them about the key developments in biosciences, especially in biotechnology. Your support will help us in our mission to feed the world with knowledge. You can help by donating as little as $10.
See more articles:
News from Around the World
- FAO Seeks More Support from G20 to Family Farmers to Meet Food Demands
- Study Shows Positive Impacts of GM Cotton Adoption in Burkina Faso
- Genetic History of Cocoa in Brazil Uncovered
- USDA Proposes Revisions on GE Crop Regulations
- New Genetic Engineering Technique to Help Design and Study Biological Systems
- Australia's OGTR Approves Field Trials of GM Cotton
- Overexpression of Wheat Ferritin Gene Enhances Heat and Abiotic Stress Tolerance
- Rice Nicotianamine Synthase 2 Improves Iron and Zinc Levels in Wheat Grains
Beyond Crop Biotech
- Researchers Produce Human Hormone Precursor in Pichia pastoris
- India Launches Friendly™ Aedes Project to Combat Vector Borne Diseases
From the BICs
- SEARCA BIC Briefs Public School Officials on Course Offerings in Agri and Biotech
- 17th Euro Biotechnology Congress
- VIB Fact Series: Golden Rice
- Targeted Multi-allelic Mutagenesis in Tetraploid Potato using CRISPR-Cas9
- DNA-free Genome Editing of Bread Wheat using CRISPR/Cas9 Ribonucleoprotein Complex
Read the latest:
- Biotech Updates (November 29, 2023)
- Gene Editing Supplement (November 29, 2023)
- Gene Drive Supplement (February 22, 2023)
Subscribe to BU: