Transgenic Jatropha curcas Boasts Geminivirus Resistance
October 29, 2014http://www.biotechnologyforbiofuels.com/content/pdf/s13068-014-0149-z.pdf
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Jatropha curcas is a promising feedstock for biodiesel production. However, it is susceptible to the geminivirus Indian cassava mosaic virus (ICMV) which severely limits productivity. Hence, the development of virus-resistant J. curcas is crucial. A team led by Nam-Hai Chua, from Rockefeller University, generated transgenic J. curcas plants that expresses an RNA homolog of the key genes of ICMV strain DNA-A, which silenced the viral genes, conferring ICMV resistance.
The efficacy and heritability of resistance was evaluated after two rounds of inoculation to prove that the progeny of transgenic plants were resistant to an ICMV strain. PCR analysis showed that transgenic lines had virus resistance. These transgenic J. curcas plants may now be used in various breeding programs.
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