Construction of Calibrator Plasmid and Nested PCR Assay for HT Soybeans
October 30, 2013| |
Northeast Agricultural University scientist Youwen Qiu and colleagues constructed a multiple-target plasmid designated as pMD18-HT-Soybean, comprising part of a junction region of genetically modified soybean events A2704-12, A5547-127, MON89788 and GTS-40-3-2, and the endogenous soybean-specific lectin gene. The limit of detection for quantification of these four event-specific genes using pMD18-HT-Soybean plasmid was 20 copies. The team also developed a nested PCR detection method for the four GM soybean events. The results confirmed that the plasmid could be used as a substitute to genomic DNA as a calibrator for the quantification of the four soybean events in food and feed products. The results also indicated that the developed nested PCR method can be used for identification and quantification of four GM soybean events and its derivates.
Read the abstract at http://link.springer.com/article/10.1007/s00217-013-2079-6.
| |
Biotech Updates is a weekly newsletter of ISAAA, a not-for-profit organization. It is distributed for free to over 22,000 subscribers worldwide to inform them about the key developments in biosciences, especially in biotechnology. Your support will help us in our mission to feed the world with knowledge. You can help by donating as little as $10.
-
See more articles:
-
News from Around the World
- Certain Policies Affect the Use of Plant Genetic Resources, Study Reveals
- Africa Rice Congress Ends with Call for Increased Investment, Benefits for Smallholders
- Scientists Studying Pathways that Control Plant Growth
- 2013 WFP Laureate Donates Award to Women Plant Science Scholars
- Malaysians' Attitude to GM Foods and Medicine
- Farmers Need to Use Latest Technologies to Increase Yield
- 60th Anniversary of DNA Structure Discovery Focuses on GM Technology
- Chinese University Holds GM Rice Taste Testing
- ICRISAT Launches Projects to Develop Improved Varieties of Legumes in India
- Report Shows Spain Continues Expansion of GE Corn Plantings
- Study Shows How Plants Tell Time
- Role of Wild Grasses on Initiating Leaf Blotch in Barley Crops Revealed
- EFSA Still Validates Previous Opinions on GM Potato EH92-527-1
- Leeds Scientists Finds Anomaly in Model Plant Arabidopsis
-
Research Highlights
- Dissipation of Cry1Ac and its Toxicity to NT Aquatic Organisms
- Construction of Calibrator Plasmid and Nested PCR Assay for HT Soybeans
-
Beyond Crop Biotech
- Genetics for Saving Coral Reefs from Climate Change
- USDA Discovers Safer FMD Vaccine
-
Announcements
- Plant Genomics Congress
-
Resources
- 40 Chances: Finding Hope in a Hungry World
-
Read the latest: - Biotech Updates (April 30, 2025)
- Gene Editing Supplement (April 30, 2025)
- Gene Drive Supplement (February 22, 2023)
-
Subscribe to BU: - Share
- Tweet