Construction of a Super Plasmid with 10 Targets GM Crop DetectionOctober 31, 2012
Standard reference molecules are important in the detection of GM crops and products. Thus, reference molecules should also advance hand in hand with the fast progress in the development of biotech crop. Xiumin Wand from the Chinese Academy of Agricultural Sciences, together with other scientists, developed a reference plasmid with ten targets from GM soybean, maize, and cotton based on another plasmid (pTLE8) which has eight targets only from GM soybean and cotton.
The research team fused three target segments of the Bt176 event-specific 3'junction (Bt176G3'), MON810 event-specific 3-junction (MON810G3') and the endogenous maize Hmg genes, into 890 bp fragment through overlap extension polymerase chain reaction (PCR). They replaced the CP4 EPSPS gene in the plasmid pTLE8 with the fusion fragment, producing a new plasmid labeled as pTLH10 with ten target genes from GM soybean, maize, and cotton. Analysis of the new plasmid's efficiency showed that it can be used to identify GM maize, and would be useful for developing identification management for various GM crops components.
Read more about the study at http://www.sciencedirect.com/science/article/pii/S0147619X12001059.
The Crop Biotech Update is a weekly newsletter of ISAAA, a non-for-profit organization. The CBU is distributed for free to over 23,000 subscribers worldwide to inform them about the key developments in biosciences, especially in agricultural biotechnology. Your support will help us in our mission to feed the world with knowledge. You can help by donating as little as $10.
See more articles:
News from Around the World
- FAO, Brazil Sign Agreement to Transfer Cotton Technology in Developing Countries
- Monsanto Offers Royalty Free Access to Technology
- Zimbabwe to Produce More Drought Resistant Crops
- Kenya Develops Maize-bug Resistant Seeds
- Paarlberg Urges Use of Biotech
- Researchers Create Potatoes with Higher Levels of Carotenoids
- Canada Approves Dicamba Tolerant Soybeans
- Glyphosate Tolerant Canola to be Released in Canada
- AAAS: Legally Mandating GM Food Labels Could Mislead Consumers
- Agricultural Economists on Bt Cotton Impacts in India
- Scientists Develop Imaging Technique that Shows Plants' Real-Time Ionic Movement
- Syngenta Forms Rice Bowl Index Advisory Board
- EFSA's Review of Plants Developed using ZFN-3
- Scientists Discover Genetic Factor that Makes Barley Plants Resistant to Salt
- Study on High Dose/Refuge Strategy for IRM of Bt Plants with Single or Pyramided Genes
- Construction of a Super Plasmid with 10 Targets GM Crop Detection
Beyond Crop Biotech
- Scientists Develop Cow's Milk against HIV
- UKM Scientist Finds Algae that Inhibits and Kills Cancer Cells
- Workshop on Agricultural Adaptations to Climate Change
- PhD Opportunities for Bioscience Graduates
- Status Report of Jackfruit Improvement in the Asia Pacific Region
- Experiences in Sub-Saharan Africa with GM Crop Risk Communication
Subscribe to CBU: