Species: Farmed Carp (Labeo rohita)

Country of 1st Author: India
Year: 2016

Institute	Central Institute of Freshwater Aquaculture Bhubaneswar 751 002, Odisha IN
Editing Method	Embryo edit
SDN Type	SDN-2
Delivery	LrTLR22Cas9 (33.3 pg) and linearized (ScaI digested) LrTLR22HR (66.3 pg) DNA constructs t were co-injected into rohu embryos at 1- to 4-cell stage
Foreign DNA	Yes
Undergone Regulatory Process	No

Target Gene(s)

Gene Name	Gene Trait	Trait Category	NGT	Methods/Remarks
TLR22 Toll-like receptor 22	Disease Resistance	Biotic Stress	CRISPR Cas9 (spCas9)

References, Documents, and Links

• Establishing targeted carp TLR22 gene disruption via homologous recombination using CRISPR/Cas9