Species: Channel catfish (Ictalurus punctatus)

Country of 1st Author: United States
Year: 2017

Institute	Auburn University Auburn, AL 36849 US
Editing Method	Embryo edit
SDN Type	SDN-1
Delivery	Fertilized eggs were injected according to the procedures for zygote injection. One-cell embryos were injected through 15–90?minutes post-fertilization and just before the beginning of the first cell division
Foreign DNA	No
Undergone Regulatory Process	No

Target Gene(s)

Gene Name	Gene Trait	Trait Category	NGT	Methods/Remarks
MSTN Myostatin	Double muscle	Yield	CRISPR Cas9 (spCas9)	Four sets of injection solutions were prepared; three by mixing each individual sgRNA with Cas9 protein separately, and the fourth by combining the three sgRNAs together with Cas9 protein

References, Documents, and Links

• Generation of Myostatin Gene-Edited Channel Catfish (Ictalurus punctatus) via Zygote Injection of CRISPR/Cas9 System