Species: Chicken (Gallus gallus)

Country of 1st Author: Australia
Year: 2023

Institute	Hendrix Genetics Research & Technology Services (Hendrix Genetics RTS) NL
Editing Method	Other
SDN Type	SDN-3
Delivery	The repair template carrying the marker gene for site-specific integration is delivered as double-stranded DNA (as a plasmid) that is introduced into the primordial germs cells by lipofection via a patented method.
Foreign DNA	Yes
Undergone Regulatory Process	No

Target Gene(s)

Gene Name	Gene Trait	Trait Category	NGT	Methods/Remarks
Site directed integration for null-segregant technology	Marker gene locked into the male sex determining chromosome for Marker Assisted Sex Sorting (fluorescent protein gene) with null segregant (non-GMO) female progeny as the outcome.	Reproduction	CRISPR Cas9 (spCas9)	CRISPR/Cas9 directed homology mediated end joining (HMEJ) to mediate a targeted break in the Z chromosome to enable site specific integration of a marker gene. The marker gene is later segregated remaining with male hatchlings and not found in the female hatchlings.

References, Documents, and Links

• Advances in genetic engineering of the avian genome: "Realising the promise"
• Potential benefits of gene editing for the future of poultry farming
• Potential benefits of gene editing for the future of poultry farming
• Sex selection in layer chickens