Species: Pig (Sus scrofa domesticus)

Country of 1st Author: Japan
Year: 2019

Institute	Tokushima University 2272-1 Ishii, Myozai-gun, Tokushima 779-3233 JP
Editing Method	Embryo edit
SDN Type	SDN-1
Delivery	three gRNA using electroporation
Foreign DNA	No
Undergone Regulatory Process	No

Target Gene(s)

Gene Name	Gene Trait	Trait Category	NGT	Methods/Remarks
CD163 Cluster of Differentiation 163	Resistance against Porcine reproductive and respiratory syndrome (PRRS)	Biotic Stress	CRISPR Cas9 (spCas9)	CRISPR/Cas9 system [guide RNAs (gRNAs) withCas9 protein] targeting theCD163gene into in vitro-fertilized porcine zygotes by electroporation

References, Documents, and Links

• Generation of CD163-edited pig via electroporation of the CRISPR/Cas9 system into porcine in vitro-fertilized zygotes