Species: Common Carp (Cyprinus carpio)

Country of 1st Author: China
Year: 2020

Breed Strain	white-black Oujiang color
Institute	Shanghai Ocean University Shanghai 201306 CN
Editing Method	Embryo edit
SDN Type	SDN-1
Delivery	A mixture of Cas9 protein (400 ng/µL) and sgRNA (225 ng/µL) was co-injected into the one-celled stage embryos (1 µL/embryo).
Foreign DNA	No
Undergone Regulatory Process	No

Target Gene(s)

Gene Name	Gene Trait	Trait Category	NGT	Methods/Remarks
MC1R Melanocortin 1 Receptor	This intronless gene encodes the receptor protein for melanocyte-stimulating hormone (MSH)	Colour	CRISPR Cas9 (spCas9)	In common carp genome, MC1R contains only one exon. Target areas on the exon of MC1R were selected ; and sgRNAs were designed using an online program (http://zifit.partners.org/ZiFiT/CSquare9Nuclease.aspx).

References, Documents, and Links

• Shrunk and scattered black spots turn out due to MC1R knockout in a white-black Oujiang color common carp (Cyprinus carpio var. color)