Species: Cattle (Bos taurus x Bos taurus indicus)

Country of 1st Author: United States
Year: 2023

Breed Strain	Gir- Holstein
Institute	US Meat Animal Research Center, United States Department of Agriculture (USDA) Clay Center, NE US
Editing Method	Cloned cell line
SDN Type	SDN-2
Delivery	65 pmol of gRNA, 78 pmol of Alt-R S.p. HiFi Cas9 Nuclease V3, and 0.11 nmol of the ssODN were added to the cells.
Foreign DNA	Yes
Undergone Regulatory Process	No

Target Gene(s)

Gene Name	Gene Trait	Trait Category	NGT	Methods/Remarks
CD46 Cluster of Differentiation 46	Resistance to Bovine Viral Diarrhoea Virus (BVDV)	Biotic Stress	CRISPR Cas9 (spCas9)	BVDV binding domain of bovine CD46

References, Documents, and Links

• First gene-edited calf with reduced susceptibility to a major viral pathogen