Species: Cattle (Bos taurus)

Country of 1st Author: United States
Year: 2020

Breed Strain	Holstein-Friesian
Institute	University of Maryland College Park, MD 20742 US
Editing Method	Embryo edit
SDN Type	SDN-1
Delivery	Presumptive zygotes were microinjected with a mixture of commercially sourced Cas9 RNP (Cas9 protein and sgRNA) and single-stranded DNA using a FemtoJet microinjector
Foreign DNA	No
Undergone Regulatory Process	No

Target Gene(s)

Gene Name	Gene Trait	Trait Category	NGT	Methods/Remarks
PRNP prion protein	Resistance to prion diseases	Biotic Stress	CRISPR Cas9 (spCas9)	0.2?µM of Cas9 protein with 0.2?µM of sgRNA and 1?µM targeting oligo

References, Documents, and Links

• One-Step Homology Mediated CRISPR-Cas Editing in Zygotes for Generating Genome Edited Cattle