Species: Cattle (Bos taurus)

Country of 1st Author: United States
Year: 2022

Institute	University of California, Davis US
Editing Method	Embryo edit
SDN Type	SDN-1
Delivery	RNPs were delivered via cytoplasmic microinjection of single-cell bovine embryos
Foreign DNA	No
Undergone Regulatory Process	No

Target Gene(s)

Gene Name	Gene Trait	Trait Category	NGT	Methods/Remarks
NANOS3 Nanos C2HC-Type Zinc Finger 3	germline ablation	Reproduction	CRISPR Cas9 (spCas9)	Dual gRNA CRISPR/Cas9 system to knockout NANOS3 using NHEJ

References, Documents, and Links

• Production of a germline ablated bull using CRISPR/Cas9 to target NANOS3 in bovine zygotes