Species: Cattle (Bos taurus)

Country of 1st Author: Japan
Year: 2017

Breed Strain	Japanese Black cattle
Institute	Institute of Agrobiological Sciences, NARO Ikenodai 2, Tsukuba, Ibaraki, 305-8602 JP
Editing Method	Cloned cell line
SDN Type	SDN-2
Delivery	donor DNA that contained a synonymous codon for the correct amino acid and an Aequorea coerulescens Green Fluorescent Protein (AcGFP) cassette with a piggyBac transposase recognition site
Foreign DNA	Yes
Undergone Regulatory Process	No

Target Gene(s)

Gene Name	Gene Trait	Trait Category	NGT	Methods/Remarks
IARS Isoleucyl-tRNA synthetase	Isoleucyl-tRNA synthetase (IARS) syndrome protection	Biotic Stress	CRISPR Cas9 (spCas9)	single nucleotide substitution

References, Documents, and Links

• Correction of a Disease Mutation using CRISPR/Cas9-assisted Genome Editing in Japanese Black Cattle