Species: Honeybee (Apis mellifera)

Country of 1st Author: Japan
Year: 2018

Institute	Department of Biological Sciences, Graduate School of Science, The University of Tokyo Bunkyo-ku, Tokyo 113-0033 JP
Editing Method	Embryo edit
SDN Type	SDN-1
Delivery	microinjecting sgRNA and Cas9 protein into the region of zygote formation within 2 hr after queen oviposition, which allows one-step generation of biallelic knockout mutants in honeybee with high efficiency.
Foreign DNA	No
Undergone Regulatory Process	No

Target Gene(s)

Gene Name	Gene Trait	Trait Category	NGT	Methods/Remarks
mKast middle-type Kenyon cell-preferential arrestin-related gene-1	Middle-type Kenyon cell-preferential arrestin-related protein (mKast)	Other	CRISPR Cas9 (spCas9)

References, Documents, and Links

• mKast is dispensable for normal development and sexual maturation of the male European honeybee