Species: Horse (Equus caballus)

Country of Determination: Argentina
Year: 2025

Developer	Kheiron Biotech AR
Editing Method	Cloned cell line
SDN Type	SDN-1
Delivery	nucleofected horse fetal fibroblasts with 1, 2 or 5 µg of 2 different gRNA/Cas9 plasmids targeting the first exon of MSTN.Â
Foreign DNA	No
Undergone Regulatory Process	Yes

Target Gene(s)

Gene Name	Gene Trait	Trait Category	NGT	Methods/Remarks
MSTN Myostatin	Double muscle	Yield	CRISPR Cas9 (spCas9)	CRISPR was used in fetal fibroblasts (connective tissue cells) to generate embryos through cloning, and then the embryos were implanted into mares

Regulatory Documents and Links

• Scientific Reports