Species: Sheep (Ovis aries)

Country of 1st Author: United States
Year: 2022

Institute	University of California, Davis US
Editing Method	Embryo edit
SDN Type	SDN-1
Delivery	Electroporation of dual-guide RNP into zygotes
Foreign DNA	No
Undergone Regulatory Process	No

Target Gene(s)

Gene Name	Gene Trait	Trait Category	NGT	Methods/Remarks
SOCS2 suppressor of cytokine signaling 2	High growth	Yield	CRISPR Cas9 (spCas9)	Dual-guide RNP targeting SOCS2
PDX1 Pancreatic And Duodenal Homeobox 1	The protein encoded by this gene is a transcriptional activator of several genes, including insulin, somatostatin, glucokinase, islet amyloid polypeptide, and glucose transporter type 2.	Other	CRISPR Cas9 (spCas9)

References, Documents, and Links

• Single-Step Genome Editing of Small Ruminant Embryos by Electroporation