Species: Atlantic Salmon (Salmo salar)

Country of 1st Author: Norway
Year: 2019

Institute	Norwegian University of Science and Technology, Department of Biology Trondheim, N-7491 NO
Editing Method	Embryo edit
SDN Type	SDN-1
Delivery	Atlantic salmon eggs were fertilized with sperm in fresh water containing 0.5?mM reduced glutathione and incubated at 6–8?°C for 2–3?hours until the first cell was visible48. Eggs were held on concavities of plastic petri dish and injected with a mixture of 50?ng/µl gRNA each for slc45a2 and elovl2, and 150?ng/µl Cas9 mRNA in Hepes buffer using the picospritzer III
Foreign DNA	No
Undergone Regulatory Process	No

Target Gene(s)

Gene Name	Gene Trait	Trait Category	NGT	Methods/Remarks
elovl2	Improves n-3 Fatty Acid Content	Quality	CRISPR Cas9 (spCas9)
slc45a2 solute carrier family 45 member 2		Colour	CRISPR Cas9 (spCas9)

References, Documents, and Links

• CRISPR/Cas9-mediated ablation of elovl2 in Atlantic salmon (Salmo salar L.) inhibits elongation of polyunsaturated fatty acids and induces Srebp-1 and target genes