Species: Atlantic Salmon (Salmo salar)

Country of 1st Author: Norway
Year: 2019

Institute	Norwegian University of Science and Technology, Department of Biology Trondheim, N-7491 NO
Editing Method	Embryo edit
SDN Type	SDN-1
Delivery	Salmon eggs were fertilized with sperm in freshwater supplemented with 0.5?mM reduced glutathione at 6–8?°C43 and incubated at 6–8?°C for 2–3?h. Embryos were microinjected with 150?ng/µl of Cas9 mRNA and a mixture of two gRNAs each 50?ng/µl in Hepes buffer using the picospritzer III
Foreign DNA	No
Undergone Regulatory Process	No

Target Gene(s)

Gene Name	Gene Trait	Trait Category	NGT	Methods/Remarks
Δ6fads2-a	Omega-3 Metabolism.	Quality	CRISPR Cas12a (Cpf1)
Δ6fads2-b	Omega-3 Metabolism	Quality	CRISPR Cas12a (Cpf1)
Δ6fads2-c	Omega-3 Metabolism	Quality	CRISPR Cas9 (spCas9)
Δ5fads2	Omega-3 Metabolism	Quality	CRISPR Cas9 (spCas9)
slc45a2 solute carrier family 45 member 2		Colour	CRISPR Cas9 (spCas9)

References, Documents, and Links

• CRISPR/Cas9-mediated editing of Δ5 and Δ6 desaturases impairs Δ8-desaturation and docosahexaenoic acid synthesis in Atlantic salmon (Salmo salar L.)