Species: Pig (Sus scrofa domesticus)

Country of 1st Author: Germany
Year: 2021

Institute	Friedrich-Loeffler-Institut Mariensee, 31535 Neustadt am Rübenberge DE
Editing Method	Embryo edit
SDN Type	SDN-1
Delivery	A total of 31 or 32 embryos derived from intracytoplasmic microinjection of the Cas9 protein and gRNA SRY_1 and SRY_3 into in vitro fertilization (IVF) produced zygotes were surgically transferred into each of three hormonally synchronized recipient sows.
Foreign DNA	No
Undergone Regulatory Process	No

Target Gene(s)

Gene Name	Gene Trait	Trait Category	NGT	Methods/Remarks
Tva	Resistance to Avian Leukosis Virus Subgroups A and K	Biotic Stress	CRISPR Cas9 (spCas9)

References, Documents, and Links

• Knock-Out of Retrovirus Receptor Gene Tva in the Chicken Confers Resistance to Avian Leukosis Virus Subgroups A and K and Affects Cobalamin (Vitamin B12)-Dependent Level of Methylmalonic Acid