Species: Pig (Sus scrofa domesticus)

Country of 1st Author: China
Year: 2019

Breed Strain	Liang Guang Small Spotted
Institute	Yat-sen University Guangzhou, 510006 CN
Editing Method	Cloned cell line
SDN Type	SDN-1
Delivery	PEFs were harvested, counted, and 1?×?106 cells were resuspended in 100 µL of buffer R (Invitrogen, USA), that contains 5 µg of RGS reporter plasmid and 5 µg of each pX330-IGF2-sgRNA plasmid. The mixture was then transfected by electroporation at 1650 V for 10 ms in 3 pulses, using the Neon Transfection System
Foreign DNA	No
Undergone Regulatory Process	No

Target Gene(s)

Gene Name	Gene Trait	Trait Category	NGT	Methods/Remarks
IGF2 Insulin-like growth factor 2	Muscle development	Yield	CRISPR Cas9 (spCas9)	SNP within intron 3 of IGF2 disrupted a binding site for the repressor, zinc finger BED-type containing 6 (ZBED6)

References, Documents, and Links

• Disruption of the ZBED6 binding site in intron 3 of IGF2 by CRISPR/Cas9 leads to enhanced muscle development in Liang Guang Small Spotted pigs