Species: Pig (Sus scrofa domesticus)

Country of 1st Author: China
Year: 2018

Breed Strain	Chinese Bama pigs
Institute	Chinese Academy of Sciences, Beijing CN
Editing Method	Embryo edit
SDN Type	SDN-1
Delivery	After confirming that the Cas9 nickase, mRNA, and sgRNAs were functional, we injected them into naturally fertilized Bama embryos and transferred total of 19 embryos into two recipients
Foreign DNA	No
Undergone Regulatory Process	No

Target Gene(s)

Gene Name	Gene Trait	Trait Category	NGT	Methods/Remarks
IGF2 Insulin-like growth factor 2	Muscle development	Yield	CRISPR Cas9 (spCas9)	Nickase mRNA and sgRNAs were injected into the cytoplasm of parthenogenetic activated embryos and zygotes using a FemtoJet microinjector

References, Documents, and Links

• Editing porcine IGF2 regulatory element improved meat production in Chinese Bama pigs