Species: Pig (Sus scrofa domesticus)

Country of 1st Author: China
Year: 2023

Breed Strain	Large White x Landrace
Institute	Agricultural Technology Research Institute Xiangshan Dist., Hsinchu TW
Editing Method	Embryo edit
SDN Type	SDN-1
Delivery	Directive microinjection of two gRNAs and Cas9 mRNA into the cytoplasm of pronuclear zygotes efficiently generated four piglets confirmed as CD163 knockout (KO) and/or CD163 exon 7 deleted (CD163?E7)
Foreign DNA	No
Undergone Regulatory Process	No

Target Gene(s)

Gene Name	Gene Trait	Trait Category	NGT	Methods/Remarks
CD163 Cluster of Differentiation 163	Resistance against Porcine reproductive and respiratory syndrome (PRRS)	Biotic Stress	CRISPR Cas9 (spCas9)	two guide RNAs (gRNAs) located on the proximal front and post sites of exon 7.

References, Documents, and Links

• Activated macrophages of CD 163 gene edited pigs generated by direct cytoplasmic microinjection with CRISPR gRNA/Cas9 mRNA are resistant to PRRS virus assault