Species: Pig (Sus scrofa domesticus)

Country of 1st Author: United States
Year: 2017

Institute	University of Missouri Columbia, Missouri US
Editing Method	Embryo edit
SDN Type	SDN-3
Delivery	The CD163L1 SRCR 8 peptide sequence was created by making 38-nucleotide substitutions that produced 31-amino-acid changes to porcine exon 7, as shown in Fig. 1B. A neomycin cassette was included in the synthesized exon to enable screening for the modification.
Foreign DNA	Yes
Undergone Regulatory Process	No

Target Gene(s)

Gene Name	Gene Trait	Trait Category	NGT	Methods/Remarks
CD163 Cluster of Differentiation 163	Resistance against Porcine reproductive and respiratory syndrome (PRRS)	Biotic Stress	CRISPR Cas9 (spCas9)	KO or Replacement of Porcine CD163 Scavenger Receptor Cysteine-Rich Domain 5 with a CD163-Like Homolog

References, Documents, and Links

• Replacement of Porcine CD163 Scavenger Receptor Cysteine-Rich Domain 5 with a CD163-Like Homolog Confers Resistance of Pigs to Genotype 1 but Not Genotype 2 Porcine Reproductive and Respiratory Syndrome Virus