Species: Yellow catfish (Pelteobagrus fulvidraco)

Country of 1st Author: China
Year: 2018

Institute	Huazhong Agricultural University Wuhan, 437000, Hubei CN
Editing Method	Embryo edit
SDN Type	SDN-3
Delivery	Cas9 mRNA (300?ng/mL) and gRNA (20?ng/mL) were co-injected into one-cell-stage embryos
Foreign DNA	Yes
Undergone Regulatory Process	No

Target Gene(s)

Gene Name	Gene Trait	Trait Category	NGT	Methods/Remarks
pfpdz1	Overexpression of pfpdz1 using additive transgenesis induces XX ovary to differentiate into testis-like tissue, while the targeted inactivation of pfpdz1 in Y chromosome using CRISPR/Cas9-mediated mutagenesis triggers ovarian differentiation.	Reproduction	CRISPR Cas9 (spCas9)

References, Documents, and Links

• A novel PDZ domain-containing gene is essential for male sex differentiation and maintenance in yellow catfish (Pelteobagrus fulvidraco)