Species: Olive flounder (Paralichthys olivaceus)

Country of 1st Author: China
Year: 2021

Institute	University of Chinese Academy of Sciences Beijing, 100049 CN
Editing Method	Embryo edit
SDN Type	SDN-1
Delivery	Electroporation. 0.5 µg gRNA1, 0.5 µg gRNA2 and 1.0 µg Cas9 mRNA for myomaker (two gRNAs), and 0.7 µg gRNA and 1.4 µg Cas9 mRNA for gsdf (one gRNA) were respectively mixed in 100 µL 1?×?PBS for electroporating.
Foreign DNA	No
Undergone Regulatory Process	No

Target Gene(s)

Gene Name	Gene Trait	Trait Category	NGT	Methods/Remarks
Myomaker		Yield	CRISPR Cas9 (spCas9)
gsdf Gonadal soma-derived factor		Reproduction	CRISPR Cas9 (spCas9)

References, Documents, and Links