Species: Sheep (Ovis aries)

Country of 1st Author: China
Year: 2023

Breed Strain	Hu
Institute	Jiangsu Provincial Engineering Research Center for Precision Animal Breeding Nanjing 210014 CN
Editing Method	Embryo edit
SDN Type	SDN-1
Foreign DNA	No
Undergone Regulatory Process	No

Target Gene(s)

Gene Name	Gene Trait	Trait Category	NGT	Methods/Remarks
MSTN Myostatin	Double muscle	Yield	CRISPR Cas9 (spCas9)	C-CRISPR simultaneously uses three to four neighboring gRNAs spaced 10–200 bp apart to target a single key exon of the target gene.

References, Documents, and Links

• Efficient and Specific Generation of MSTN-Edited Hu Sheep Using C-CRISPR