Species: Nile tilapia (Oreochromis niloticus)

Country of 1st Author: China
Year: 2016

Institute	Southwest University 400715 Chongqing CN
Editing Method	Embryo edit
SDN Type	SDN-1
Delivery	The 5'-capped mRNAs (200 pg each) generated with in vitro transcription using the mMESSAGE mMACHINE SP6 Kit (Invitrogen, USA) were microinjected into one-cell-stage embryos
Foreign DNA	No
Undergone Regulatory Process	No

Target Gene(s)

Gene Name	Gene Trait	Trait Category	NGT	Methods/Remarks
RSPO1 R-spondin 1	RSPO1 encodes a secreted signaling molecule that regulates WNT4 expression and is essential for female sex determination	Reproduction	CRISPR Cas9 (spCas9)

References, Documents, and Links